Poietis provides industrial stakeholders and researchers with a unique platform to design and manufacture bio-printed products for regenerative medicine, preclinical research and evaluating the efficacy of cosmetic products and ingredients.
The unquestionable added value of the Poietis platform is its ability to design and manufacture tissue with cellular resolution. This allows very precise control of the manufacturing process and guarantees the reproducibility and reliability of bioprinted products.
The platform contains three parts: the design, printing and maturation of biological tissues.
Bioprinting has specific requirements that are directly linked to the nature of cells, printed biomaterials and the multi-scale aspects of the mechanisms underpinning the self-organization of tissue. To understand them, Poietis has developed a specific “cytocentric” CAD tool. This tool makes it possible to define the position and the local environment of each of the cells within three-dimensional tissue structures by integrating several cell types and different materials.
The digital file thus generated describes the architecture of the biological tissues with the 3D organization of the tissue’s constituents (cells and extracellular matrix).
3D printing of biological tissues is performed by layer-by-layer deposition of biological ink microdroplets containing one to several cells and extracellular matrix.
The NGB 17.03 is a new-generation bio-printer capable of 3D-printing each cell and its matrix in sterile conditions, with great accuracy and reliability thanks to its eight-axis motion capacity and multiple real-time manufacturing control systems.
Maturation, also called morphogenesis, is the period during which the processes of tissue self-organization occur (cellular communication, cell-extracellular matrix interactions). Thanks to a specific imaging system, it is possible to monitor the progression of the migration, proliferation and differentiation of the cells.
Examples of Poietis bioprinted skin
At Poietis, we are developing a 4D bioprinting approach which consists in programming tissue self-organization by designing tissue constituent organizations (cells and extracellular matrix) that evolve in a controlled way until specific biological functions emerge. Thus, by analyzing tissue evolution during maturation, we are able to optimize the initial tissue architecture defined by CAD in order to improve the functionality of the printed tissues and guarantee that they are manufactured in the most reliable way.
Because it is the only way to guarantee the reproducibility and reliability of bioprinted tissues.
Conventional bioprinting technologies cannot be used to reliably control the deposition of cells because they are deposited randomly. This means that the reproducibility and reliability of bioprinted tissues cannot be guaranteed. This would represent an unacceptable hurdle, not only for industrial production but also in terms of regulatory requirements for future clinical applications.
Poietis provides the solution with the first single-cell bioprinting platform that allows the design and manufacture of biological tissues by controlling both the resolution (the ability to print cell by cell) and the accuracy of printing (the ability to precisely position the cell in a 3D environment).
Unlike conventional approaches to tissue engineering or extrusion bioprinting, laser-assisted bioprinting allows cells to be positioned in three dimensions with micrometric resolution and precision. It is therefore the bioprinting technology that offers the highest resolution.
Laser-assisted bioprinting makes it possible to print cell by cell according to the following physical mechanism. The focusing of a laser pulse (in blue) on a cartridge (composed of an ink film spread on a glass plate) results in the formation of an ink jet towards a substrate on which cell microdroplets are collected. By controlling the physical conditions of the ejection (energy, viscosity...), the volume of the droplets is controlled precisely (~ picoliter). The cell patterns are obtained by rapid scanning of the cartridge by the laser, which results in the formation of 10,000 droplets per second.